Hollenberg Supplement Mixture
For use with Hollenberg's modification of Field's two hybrid system for HIS selection; see reference 7, pg 158. Use HSM in conjunction with DOB w/ Succinate (Cat. No. 4025-622) on page 276, and with agar pg 277.
| Supplement | mg/L | Supplement | mg/L |
| Adenine | 100 | Phenylalanine | 50 |
| Argenine | 100 | Proline | 50 |
| Aspartic Acid | 50 | Serine | 50 |
| Cysteine | 100 | Threonine | 100 |
| Histidine | 50 | Tryptophan | 100 |
| Isoleucine | 50 | Tyrosine | 50 |
| Leucine | 100 | Uracil | 100 |
| Lysine | 100 | Valine | 50 |
| Methionine | 50 | Total | 1250 |
| Quantity | Cat. No. | g/Liter | |
| HSM-LEU | |||
|---|---|---|---|
| Powder | |||
| 50 g | 4610-012 | 1.15 | |
| HSM-TRP-URA | |||
| Powder | |||
| 50 g | 4620-012 | 1.05 | |
| HSM-LEU-URA | |||
| Powder | |||
| 50 g | 4620-112 | 1.05 | |
| HSM-MET-TRP-URA | |||
| Powder | |||
| 50 g | 4630-012 | 1.00 | |
| HSM-LEU-TRP-URA | |||
| Powder | |||
| 50 g | 4630-112 | 0.95 | |
| HSM-HIS-LEU-LYS-TRP-URA | |||
| Powder | |||
| 50 g | 4650-012 | 0.80 | |