Complete Supplement Mixture (CSM)
The formulation for CSM, shown below, has been time tested (ref. 6-10, pg. 273) as a dropout supplement for virtually all strains of S. cerevisiae. Cells grow vigorously in CSM supplemented medium which is easily made by adding a small quantity of the CSM powder to DOB or DOBA Base Medium and autoclaving. See page 152 for additional supplements for specific requirements by certain two hybrid system strains.
| CSM Formation | mg/liter |
|---|---|
| Adenine | 10* |
| Arginine | 50 |
| Aspartic Acid | 80 |
| Histidine | 20 |
| Isoleucine | 50 |
| Leucine | 100 |
| Lysine | 50 |
| Methionine | 20** |
| Phenylalanine | 50 |
| Threonine | 100** |
| Tryptophan | 50 |
| Tyrosine | 50 |
| Uracil | 20 |
| Valine | 140 |
| TOTAL | 790 |
| * Minimum quantity for healthy
growth and yet optimized to promote red color in certain adenine
auxotrophs. CSM formulations are available that contain 20 or 40
mg/liter of adenine. ** 80 mg/liter of Homoserine is substituted for Threonine in mixtures where Methionine is dropped-out. | |
CSM Powder Mixtures are available in 10 g and 100 g sizes. The 10 g size is sufficient to supplement >12 liters of media.
| Description | Cat No. | g/liter |
| CSM (complete mixture) | 4500-0x2 | 0.79 |
| Single Drop Out | ||
| CSM-ADE (missing Adenine) | 4510-0x2 | 0.78 |
| CSM-ARG | 4510-1x2 | 0.74 |
| CSM-ARG w/40mg/L ADE | 4512-6x2 | 0.77 |
| CSM-ASP | 4510-2x2 | 0.71 |
| CSM-HIS | 4510-3x2 | 0.77 |
| CSM-HIS w/10mg/L ALA | 4511-8x2 | 0.78 |
| CSM-HIS w/20mg/L ADE | 4512-2x2 | 0.78 |
| CSM-HIS w/25mg/L ALA | 4511-9x2 | 0.80 |
| CSM-HIS w/40mg/L ADE | 4512-8x2 | 0.80 |
| CSM-HIS w/50mg/L ALA | 4512-0x2 | 0.82 |
| CSM-Isoleucine | 4510-4x2 | 0.74 |
| CSM-LEU | 4510-5x2 | 0.69 |
| CSM-LEU w/20mg/L ADE | 4512-4x2 | 0.70 |
| CSM-LEU w/40mg/L ADE | 4511-4x2 | 0.72 |
| CSM-Lysine | 4510-6x2 | 0.74 |
| CSM-LYS w/40mg/L ADE | 4512-1x2 | 0.77 |
| CSM-MET | 4510-7x2 | 0.75 |
| CSM-PHE | 4510-8x2 | 0.74 |
| CSM-THR | 4510-9x2 | 0.69 |
| CSM-TRP | 4511-0x2 | 0.74 |
| CSM-TRP w/10mg/L ALA | 4511-5x2 | 0.75 |
| CSM-TRP w/20mg/L ADE | 4512-5x2 | 0.75 |
| CSM-TRP w/25mg/L ALA | 4511-6x2 | 0.77 |
| CSM-TRP w/50mg/L ALA | 4511-7x2 | 0.79 |
| CSM-TYR | 4511-1x2 | 0.74 |
| CSM-URA | 4511-2x2 | 0.77 |
| CSM-URA w/20mg/L ADE | 4512-3x2 | 0.78 |
| CSM-URA w/40mg/L ADE | 4512-7x2 | 0.80 |
| CSM-VAL | 4511-3x2 | 0.65 |
| Double Drop out | ||
| CSM-ADE-ARG | 4523-4x2 | 0.73 |
| CSM-ADE-HIS | 4521-9x2 | 0.76 |
| CSM-ADE-LEU | 4521-5x2 | 0.68 |
| CSM-ADE-LYS | 4521-0x2 | 0.73 |
| CSM-ADE-MET | 4523-2x2* | 0.74 |
| CSM-ADE-TRP | 4522-8x2 | 0.73 |
| CSM-ADE-URA | 4521-8x2 | 0.76 |
| CSM-ARG-HIS | 4522-0x2 | 0.72 |
| CSM-ARG-TRP | 4521-7x2 | 0.69 |
| CSM-ARG-URA | 4523-1x2 | 0.72 |
| CSM-HIS-LEU | 4520-4x2 | 0.67 |
| CSM-HIS-LEU w/20mg/L ADE | 4522-4x2 | 0.68 |
| CSM-HIS-LEU w/40mg/L ADE | 4522-9x2 | 0.70 |
| CSM-HIS-LYS | 4521-2x2 | 0.72 |
| CSM-HIS-MET | 4521-3x2* | 0.73 |
| CSM-HIS-TRP | 4520-1x2 | 0.72 |
| CSM-HIS-TRP w/40mg/L ADE | 4522-7x2 | 0.75 |
| CSM-HIS-TYR | 4523-3x2 | 0.72 |
| CSM-HIS-URA | 4520-3x2 | 0.75 |
| CSM-HIS-URA w/40mg/L ADE | 4522-6x2 | 0.78 |
| CSM-LEU-LYS | 4521-1x2 | 0.64 |
| CSM-LEU-MET | 4523-5x2* | 0.65 |
| CSM-LEU-MET w/30 mg/L SER | 4523-6x2* | 0.68 |
| CSM-LEU-THR | 4522-1x2 | 0.59 |
| CSM-LEU-TRP | 4520-0x2 | 0.64 |
| CSM-LEU-TRP w/20mg/L ADE | 4522-3x2 | 0.65 |
| CSM-LEU-TRP w/40mg/L ADE | 4523-0x2 | 0.67 |
| CSM-LEU-URA | 4520-2x2 | 0.67 |
| CSM-LEU-URA w/40mg/L ADE | 4520-7x2 | 0.70 |
| CSM-LYS-TRP | 4520-6x2 | 0.69 |
| CSM-LYS-TYR | 4521-6x2 | 0.69 |
| CSM-LYS-URA | 4521-4x2 | 0.72 |
| CSM-MET-TRP | 4520-9x2 | 0.70 |
| CSM-MET-URA | 4520-8x2 | 0.73 |
| CSM-THR-TRP | 4522-2x2 | 0.64 |
| CSM-TRP-URA | 4520-5x2 | 0.72 |
| CSM-TRP-URA w/40mg/L ADE | 4522-5x2 | 0.75 |
| Triple Drop out | ||
| CSM-ADE-ARG-LYS | 4532-3x2 | 0.68 |
| CSM-ADE-HIS-TRP | 4531-5x2 | 0.71 |
| CSM-ADE-HIS-URA | 4531-8x2 | 0.74 |
| CSM-ADE-LEU-MET | 4530-2x2* | 0.64 |
| CSM-ADE-LEU-TRP | 4532-4x2 | 0.63 |
| CSM-ADE-LEU-URA | 4531-9x2 | 0.66 |
| CSM-ADE-MET-TRP | 4532-2x2 | 0.69 |
| CSM-ADE-MET-URA | 4530-4x2* | 0.72 |
| CSM-ADE-TRP-URA | 4531-0x2 | 0.71 |
| CSM-ARG-LEU-URA | 4532-5x2 | 0.62 |
| CSM-HIS-LEU-TRP | 4530-1x2 | 0.62 |
| CSM-HIS-LEU-TRP w/40mg/L ADE | 4530-5x2 | 0.65 |
| CSM-HIS-LEU-URA | 4531-2x2 | 0.65 |
| CSM-HIS-LEU-URA w/20mg/L Ser, Pro, Cys | 4531-7x2 | 0.71 |
| CSM-HIS-LYS-TRP | 4532-0x2 | 0.67 |
| CSM-HIS-LYS-TRP w/20 mg/L CYS, PRO, SER | 4535-1x2 | 0.73 |
| CSM-HIS-LYS-URA | 4531-4x2 | 0.70 |
| CSM-HIS-MET-URA | 4530-7x2* | 0.71 |
| CSM-HIS-TRP-URA | 4530-8x2 | 0.70 |
| CSM-HIS-TRP-VAL | 4531-3x2 | 0.58 |
| CSM-LEU-LYS-TRP | 4530-3x2 | 0.59 |
| CSM-LEU-LYS-URA | 4532-6x2 | 0.62 |
| CSM-LEU-MET-TRP | 4532-1x2* | 0.60 |
| CSM-LEU-MET-URA | 4532-7x2* | 0.63 |
| CSM-LEU-TRP-URA | 4530-9x2 | 0.62 |
| CSM-LYS-TRP-URA | 4530-0x2 | 0.67 |
| CSM-LYS-TYR-URA | 4531-6x2 | 0.67 |
| CSM-MET-TRP-URA | 4530-6x2* | 0.68 |
| CSM-PHE-TRP-TYR | 4531-1x2 | 0.64 |
| Four Drop outs | ||
| CSM-ADE-ARG-MET-TYR | 4541-5x2* | 0.64 |
| CSM-ADE-HIS-LEU-LYS | 4540-7x2 | 0.61 |
| CSM-ADE-HIS-LEU-TRP | 4540-4x2 | 0.61 |
| CSM-ADE-HIS-LEU-URA | 4540-9x2 | 0.64 |
| CSM-ADE-LEU-LYS-TRP | 4540-6x2 | 0.58 |
| CSM-ADE-LEU-TRP-URA | 4540-3x2 | 0.61 |
| CSM-ADE-MET-TRP-URA | 4541-7x2* | 0.67 |
| CSM-ARG-HIS-LEU-TRP w/20mg/L CYS,PRO,SER | 4541-2x2 | 0.63 |
| CSM-ASP-HIS-LEU-TRP | 4540-8x2 | 0.54 |
| CSM-HIS-LEU-LYS-TRP | 4540-2x2 | 0.57 |
| CSM-HIS-LEU-LYS-URA | 4540-5x2 | 0.60 |
| CSM-HIS-LEU-MET-TRP | 4541-3x2* | 0.58 |
| CSM-HIS-LEU-TRP-URA | 4540-0x2 | 0.60 |
| CSM-HIS-LEU-TRP-URA w/20mg/L CYS,PRO,SER | 4541-1x2 | 0.66 |
| CSM-HIS-LYS-TRP-URA | 4541-0x2 | 0.65 |
| CSM-LEU-LYS-TRP-URA | 4540-1x2 | 0.57 |
| CSM-LEU-MET-TRP-URA | 4541-6x2* | 0.58 |
| Five Drop outs | ||
| CSM-ADE-HIS-LEU-LYS-TRP | 4550-2x2 | 0.56 |
| CSM-ADE-HIS-LEU-TRP-URA | 4550-1x2 | 0.59 |
| CSM-ADE-HIS-LYS-TRP-URA | 4550-3x2 | 0.64 |
| CSM-HIS-LEU-LYS-TRP-URA | 4550-0x2 | 0.55 |
| CSM-HIS-LEU-MET-TRP-URA | 4550-4x2* | 0.56 |
| Six Drop outs | ||
| CSM-ADE-HIS-LEU-LYS-TRP-URA | 4560-1x2 | 0.54 |
| CSM-ADE-HIS-LEU-LYS-TRP-URA w/30mg/L Serine | 4560-0x2 | 0.57 |
| CSM-ADE-HIS-LEU-MET-TRP-URA | 4560-2x2* | 0.55 |
| CSM-HIS-LEU-LYS-MET-TRP-URA | 4560-3x2* | 0.51 |
| Seven Drop outs | ||
| CSM-ADE-HIS-LEU-LYS-TRP-TYR-URA | 4570-0x2 | 0.49 |
| Eight Drop outs | ||
| CSM-ADE-ARG-HIS-LEU-MET-THR-TRP-URA | 4580-1x2 | 0.42 |
| CSM-ASP-HIS-LEU-LYS-MET-THR-TRP-URA | 4580-0x2 | 0.35 |