Brent Supplement Mixture (BSM)
"Brent Supplement Mixture" for use with the Interaction Trap two hybrid system. See references 8 and 11 on page 158 for description. BSMs are added to DOB and DOBA with dextrose, raffinose, or galactose as carbon source (see pp 276-278).
| Supplement | mg/L | Supplement | mg/L |
| Adenine | 40 | L-Phenylalanine | 50 |
| L-Argenine HCl | 20 | L-Threonine | 200 |
| L-Aspartic Acid | 100 | L-Serine | 375 |
| L-Histidine | 20 | L-Tryptophan | 40 |
| L-Isoleucine | 30 | L-Tyrosine | 30 |
| L-Leucine | 60 | Uracil | 20 |
| L-Lysine | 30 | L-Valine | 150 |
| L-Methionine | 20 | Total | 1285 |
| Quantity | Cat. No. | g/Liter | |
| BSM-URA | |||
|---|---|---|---|
| Powder | |||
| 25 g | 4710-012 | 1.265 | |
| BSM-HIS-URA | |||
| Powder | |||
| 25 g | 4720-012 | 1.245 | |
| BSM-HIS-LEU-URA | |||
| Powder | |||
| 25 g | 4730-012 | 1.185 | |
| BSM-HIS-TRP-URA | |||
| Powder | |||
| 25 g | 4730-112 | 1.205 | |
| BSM-HIS-LEU-TRP-URA | |||
| Powder | |||
| 25 g | 4740-012 | 1.145 | |