FastRNA^®, GREEN Kit
Total RNA Isolation from Plant and Animal Tissues

The FastRNA GREEN kit uses the FastPrep Instrument for rapid isolation of total RNA from plant and animal tissues or cells. The lysing matrix, which consists of zirconium particles, disrupts tissues and lyses cells during processing in the FastPrep. The presence of chaotropic reagents during lysis ensures immediate stabilization of RNA. The RNA is purified by organic extractions and precipitation with isopropanol. The kit contains enough reagents for 100 preps using 50-250 mg of tissue or 10⁷ cells and yields 20-100 ug of total RNA. The entire process is completed in less than one hour.

The protocol, in brief:

1. The following reagents are first prepared from solutions present in the kit:
   

   a) phenol acid reagent (PAR);

   b) chloroform/isoamyl alcohol (CIA);

   c) DEPC-treated isopropanol (DIPS);

   d) salt/ethanol solution (SEWS);

2. PAR, CIA and Chaotropic Stabilizing Reagents are added to a FastPrep GREEN tube containing lysing matrix;
3. Add up to 250 mg or 10⁷ cells;
4. Process in FastPrep Instrument for 20-40 seconds;
5. Spin in a microcentrifuge to separate phases;
6. Add additional CIA, vortex to mix, and centrifuge to separate phases;
7. Transfer upper phase to a separate tube and add DIPS to precipitate RNA; centrifuge;
8. Wash pellet by resuspension in SEWS and centrifugation;
9. Based on subsequent use, dissolve RNA pellet in one of three kit-supplied aqueous RNase-free solvents.

FastRNA®,  GREEN Kit
Cat. No.	Description	Size/Vol.
6020-600	FastRNA Kit GREEN	100 Preps
Kit Contents
6020-601	FastRNA Tubes with Matrix	100 Tubes
6020-602	CRSR-Green	55 ml
6020-603	*PAR	16.5 ml
6020-604	**CIA	2.5 ml
6020-605	DIPS	1 ml
6020-606	SEWS	13 ml
6020-607	SAFE	12 ml
6020-608	SAFEE	12 ml
6020-609	SSAFE	12 ml
6020-610	BBFXE	200 µl
6020-611	LiCl	5 ml
User Supplies: *phenol, **chloroform

Total RNA from Wheat
	Total RNA isolated by the FastRNA GREEN Kit from a wheat plant. From left to right: Kernel, young leaf, stem, root, apex, 2 kernels, mature leaf.