aseERASE
For stabilization of RNA and DNA solutions.

aseERASE is a proprietary matrix-based product from BIO 101 that will eliminate trace enzymatic activities from solutions of RNA and DNA. A low level of endonuclease activity is often present in RNA and DNA samples isolated from cells or tissues. With DNA this often becomes apparent only after adding the buffer to an enzyme reaction. The reaction will work fine, but the DNA will also be degraded by the buffer-activated contaminating nucleases. RNA solutions are usually stored at -70°C because the presence of any ribonuclease activity will rapidly degrade RNA. Now, it is possible to easily, quickly, and economically solve this problem.

Add aseERASE Matrix to a DNA or RNA sample and spin. Nuclease activities and other contaminants are removed leaving RNA and DNA that is stable at room temperature for long periods of time, even in the presence of Mg++, Mn++, or other activators of endonucleases.

The Protocol, in brief:

1. Add aseERASE matrix suspension to DNA or RNA sample and incubate at room temperature for 2 minutes.
2. Spin mixture in a microcentrifuge for 1 minute.
3. Transfer supernatant containing nuclease-free RNA or DNA to a new tube and discard matrix pellet.

aseERASE Matrix
Cat. No.	Treatments	Volume
2601-104	100 samples	500 ul
2601-204	250 samples	1.25 ml
2601-304	1000 samples	4 x 1.25 ml

Time at room temperature

Plasmid DNA is stable when exposed to 0.1 unit of DNase I treated with aseERASE. Lane 1: control, no DNAse. Lane 2: DNAse, before aseERASE, time 0. Lane 3: DNAse, after aseERASE, time 0. Lane 4: same as 2 at 30 minutes. Lane 5: same as 3 at 30 minutes.