GENECLEAN^® SPIN Protocol
Revision No. 1101-999-6D01W

Protocol Time: 15 minutes.

1. Melt gel in 400 µl of GC SPIN GLASSMILK in GC SPIN Filter.
2. Wash with GC SPIN NEW Wash*.
3. Elute DNA with GC SPIN Elution Solution.

For elimination of salts, proteins/enzymes, oligomers, linkers, primers, traces of detergents or organic solvents.

1. Add DNA solution to 400 µl of GC SPIN GLASSMILK in GC SPIN Filter.
2. Wash with GC SPIN NEW Wash*.
3. Elute DNA with GC SPIN Elution Solution.

Protocol Time: 15 minutes.

1. Melt gel in 400 µl of GC SPIN GLASSMILK in GC SPIN Filter.
   1. Shake to mix GC SPIN GLASSMILK and add 400 µl to GC SPIN Filter
   2. Add gel slice (300 mg maximum/filter).
   3. Heat to 55°C for 5 minutes to melt gel; flick tube to mix. Invert every minute to prevent settling of matrix.
   4. Spin liquid out of filter into catch tube. Empty catch tube as needed.
2. Wash with GC SPIN NEW Wash (add ethanol to Wash before first use).
   1. Add 500 µl of GC SPIN NEW Wash to the filter.
   2. Spin for 30 seconds or until SPIN Filter is emptied of wash.
   3. Optional: Repeat wash.
   4. Empty catch tube and spin for 1 minute to "dry" pellet.
3. Elute DNA with GC SPIN Elution Solution.
   1. Transfer SPIN Filter to a fresh catch tube.
   2. Add 10 to 25 µl GC SPIN Elution Solution and resuspend GLASSMILK by flicking the tube or by gently vortexing for 1-2 seconds.
   3. Spin for 30 seconds to transfer eluted DNA to Catch Tube.
   
   
   

   Note: A second elution can increase yield an additional 10-15%

   
   
   
   4. Discard SPIN Filter and cap the tube. DNA in solution is ready to use without further manipulation.

Protocol Time: 10 minutes.

For elimination of salts, proteins/enzymes, oligomers, linkers, primers, traces of detergents or organic solvents.

1. Add DNA solution to 400 µl of GC SPIN GLASSMILK in SPIN Filter.
   1. Shake to mix GC SPIN GLASSMILK and add 400 µl to SPIN Filter.
   2. Add DNA solution (300 µl maximum/filter). Incubate at room temperature for 5 minutes. Invert tube every minute to prevent settling of GLASSMILK
   3. Spin liquid out of SPIN Filter into catch tube. Empty catch tube Protocol Index as needed.
2. Wash with Geneclean SPIN NEW Wash (add ethanol to Wash before first use).
   1. Add 500 µl of Geneclean SPIN NEW Wash to the SPIN Filter.
   2. Spin for 30 seconds or until SPIN Filter is emptied of wash.
   3. Optional: Repeat wash.
   4. Empty Catch Tube and spin for 1 minute to "dry" pellet.
3. Elute DNA with Geneclean SPIN Elution Solution.
   1. Transfer SPIN Filter to a catch tube.
   2. Add 10 to 25 µl Geneclean SPIN Elution Solution and resuspend GLASSMILK by flicking the tube or by gently vortexing for 1-2 seconds.
   3. Spin for 30 seconds to transfer eluted DNA to catch tube. A second elution can increase yield an additional 10-15%.
   4. Discard SPIN Filter and cap the tube. DNA in solution is ready to use without further manipulation.