"Poker Face" Gel Loading Dyes
No smiles, no frowns, ready-to-load premixed dyes. Prepared and thoroughly tested for consistent results


For neutral DNA or RNA agarose gels.
General purpose loading dyes

BBS
Bromphenol blue, sucrose
Cat. No.10 x conc.
2325-1041 ml
2325-2045 ml
2325-30415 ml


BBG
Bromophenol blue, glycerol
Cat. No.10 x conc.
2327-1041 ml
2327-2045 ml
2327-30415 ml


BBF
Bromphenol blue, Ficoll®
Cat. No.10 x conc.
2329-1041 ml
2329-2045 ml
2329-30415 ml


BBXF
Bromphenol blue, xylene cyanole FF, Ficoll®
Cat. No.10 x conc.
2331-1041 ml
2331-2045 ml
2331-30415 ml
Because xylene cyanole migrates approximately one half as fast as bromphenol blue, it is useful as a tracking dye when running gels for an extended time - long after bromphenol blue has run off the bottom of the gel.


BBXG
Bromphenol blue, xylene cyanole FF, glycerol
Cat. No.10 x conc.
2333-1041 ml
2333-2045 ml
2333-30415 ml
Because xylene cyanole migrates approximately one half as fast as bromphenol blue, it is useful as a tracking dye when running gels for an extended time - long after bromphenol blue has run off the bottom of the gel.


BBXS
Bromphenol blue, xylene cyanole FF, sucrose
Cat. No.10 x conc.
2325-1041 ml
2325-2045 ml
2325-30415 ml
Because xylene cyanole migrates approximately one half as fast as bromphenol blue, it is useful as a tracking dye when running gels for an extended time - long after bromphenol blue has run off the bottom of the gel.


BBFSE
Bromphenol blue, Ficoll®, SDS, EDTA
Cat. No.10 x conc.
2337-1041 ml
2337-2045 ml
2337-30415 ml
It is often desirable to enhance dissociation of enzymes or other proteins (including whole viruses and phage) from DNA and RNA before electrophoresis. The following gel loading dye, BBFSE, contains SDS and EDTA to achieve this and is an excellent general purpose mixture for preparing nucleic acids for electrophoresis. BBFSE is also useful for disrupting adhesion of cos sites before electrophoresis of lambda phage markers so that all the fragments will form discrete bands in correct molar ratios.


BGFNE
Bromcresol green, Ficoll®, NaOH, EDTA
Cat. No.10 x conc.
2339-1041 ml
2339-2045 ml
2339-30415 ml
For alkaline agarose gels, bromcresol green is easier to see than bromphenol blue at high pH.


BBXFE
Bromphenol blue, xylene cyanole FF, Ficoll®, EDTA (RNase-free)
Cat. No.10 x conc.
2343-1041 ml
2343-2045 ml
2343-30415 ml
For formaldehyde agarose gels, add 2 µl of 10x RNase-free, BBXFE to 3 µl of 37% formaldehyde, 2 µl of 10x MOPS, pH 7, and 1 µl of 10 mg/ml ethidium bromide solution. Add 1/10 volume of the mixture to the RNA sample and heat at 50°C for 20 minutes. Run the sample on a formaldehyde gel that does not contain ethidium bromide [ref. Rosen and Villa-Komaroff 1990, Focus BRL, 12(1):23]. The RNA will stain with ethidium bromide and exhibit very little background. If ethidium bromide is added to the gel, the background fluorescence will be too high.


BBXET
Bromphenol blue, xylene cyanole FF, Ficoll®, EDTA, in 5x TBE
Cat. No.10 x conc.
2341-1041 ml
2341-2045 ml
2341-30415 ml
For non-denaturing polyacrylamide gels, the following is an excellent gel loading dye for general use.


BBXFAE
Bromphenol blue, xylene cyanole FF, deionized formamide, EDTA
Cat. No.10 x conc.
2345-1041 ml
2345-2045 ml
2345-30415 ml
For manual dideoxysequencing gels or other denaturing polyacrylamide gels use the following standard gel loading dye mixture.


FRED
Blue dextran, deionized formamide, EDTA.
Cat. No.10 x conc.
2346-1041 ml
2346-2045 ml
2346-30415 ml
For automated fluorescent sequencing gels the use of blue dextran (which will not enter the gel, unlike bromphenol blue or xylene cyanole) as a visual marker will prevent interference of the marker dye with laser detection systems.


BBXNEFA
Bromphenol blue, xylene cyanole FF, NaOH, EDTA, deionized formamide.
Cat. No.10 x conc.
2347-1041 ml
2347-2045 ml
2347-30415 ml
For chemical sequencing gels use the following standard gel loading solution;


Blue Juice
Bromphenol blue, urea, SDS, ß-mercaptoethanol.
Cat. No.2 x conc.
2400-2045 ml
2400-40425 ml
2400-604100 ml
For SDS-PAGE protein gels use the above standard dye mix;


"Green" Gel Dye
Bromphenol blue, Ficoll®, ethidium bromide.
Cat. No.6 x conc.
2410-1041 ml
2410-2045x1 ml
Decrease amount of ethidium bromide as much as 100 fold. The "Green" Gel Dye provides a significant decrease in the use of ethidium bromide; the EtBr is added to the sample only and not the gel. DNA bands are stained just as if ethidium bromide was included in the gel.






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