ssDNA Expression in pMEX Vectors
Cloning, sequencing, mutagenesis, and expression in one vector system
Optimized for Strong Gene Expression:
APPLICATIONS
The pMEX DNA vector system is easy to handle and yet provides a wide range of useful properties. The pMEX DNA cloning vectors combine the cloning and expression advantages of DNA plasmid (or cosmid) systems with the sequencing and mutagenesis advantages of single-strand phage systems. The pMEX vectors contain the pBR322 origin of replication and the phage f1 intergenic region. Thus, genes or libraries can be cloned, sequenced, mutagenized, and expressed in the same vector system. To optimize gene expression, the distance between the promoter and the ribosomal binding site (Shine-Dalgarno sequence) can easily be shortened, to the optimal length of 5 to 20 base pairs, by taking advantage of site directed mutagenesis. Given the strong promoter, this results in high levels of expression of the cloned gene product. The MCS allows easy sequencing of inserts by forming nested deletions from either end. The genetic elements (operator, promoter, start and stop codons, terminators) and both sequencing primer sequences remain unchanged. This also enables protein engineering by truncation from both sides with stable gene expression. E. coli lacIq strains can be used, which is advantageous for the expression of toxic proteins. The strong promoter does not require heat shock activation; thus no SOS response will interfere with protein expression.
| ORDER INFORMATION | ||
| Cat. No. | Description | Size/Vol. |
| 5300-810 | pMEX5 vector, f1 ori - | 10 µg |
| 5300-811 | pMEX6 vector, f1 ori + | 10 µg |
| 5300-812 | pMEX7 vector, f1 ori - | 10 µg |
| 5300-813 | pMEX8 vector, f1 ori + | 10 µg |
| 5300-814 | pMEX 5' sequencing primer | |
| 5300-815 | pMEX 3' sequencing primer | |
| Shipped at RT; store at 4°C | ||
References:
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