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In the PheBo-System for fusion protein cloning and purification, the protein of interest is cloned next to the leader protein ß-lactamase. The expressed fusion protein remains in the cytoplasm (vectors pHKcyto1 and pHKcyto2) or is released into the periplasm with the help of a signal sequence (vectors pHKperi1 and pHKperi2). ß-lactamase allows efficient protein purification under mild conditions on pre-packed re-usable Phenylboronate (PheBo-) affinity columns. The leader protein is removed by the site-specific Endoproteinase Pro-Pro-Y-Pro (Igase), which also cleaves inclusion bodies. After a second passage through the same affinity column, the protein of interest can be obtained in pure form from the column flow-through, whereas ß-lactamase and the endoproteinase remain on the column.
2 ml column, pre-packed with luer-lock connection at both ends
|Kits with 1 vector (5 µg), Endoproteinase Pro-Pro-Y-Pro (50 µg), 2 ml PheBo Column:|
|Kits with 2 vectors (5 µg each), Endoproteinase Pro-Pro-Y-Pro (50 µg), 2 ml PheBo Column:|
|Kit with all 4 vectors (5 µg each), Endoproteinase Pro-Pro-Y-Pro (50 µg), 2 ml PheBo Column:|
|5300-516||Endoproteinase Pro-PRo-Y-Pro (Igase), 50 µg|
|5300-517||Endoproteinase Pro-PRo-Y-Pro (Igase), 250 µg|
|5300-518||2 ml Phenylboronate Column|
|5300-519||5 ml Phenylboronate Column|
Vectors can be purchased separately. Vectors and columns shipped at RT; store at 4°C.|
Proteases are shipped at 4°C; store at -20°C.
A detailed handbook is provided.