Broad Host Range - Vector pBBR122
A versatile multi-copy cloning vector.
Replicated and is stable in Gram-negative Bacteria

APPLICATIONS

• express inserts in bacteria other than E. coli
• compare expression in different host cells.
• study broad host-range replicons
• enables study of a wide variety of Gram-negative bacteria

The plasmid pBBR122 is a cloning vector with a very broad-host-range. As opposed to other known broad-host-range vectors, it replicates at high copy numbers and has a small size (5304 bp). This greatly facilitates genetic studies of a wide variety of Gram-negative bacteria and makes pBBR122 particularly interesting for studies of broad-host-range replicons. Being a versatile expression vector, it is stable in all Gram-negative organisms attemped thus far. The plasmid grows under standard conditions using appropriate selective pressure.

pBBR122 was derived from pBBR1, which was isolated from Bordetella bronchiseptica S875. It is compatible with other broad-host-range vectors, since it does not belong to incompatibility groups IncP, IncQ or IncW. Being a mobilizable, but not a conjugative plasmid, pBBR122 combines the replication mechanism of Gram-negative bacteria with the mobilization mechanism of Gram-positive organisms. The Mob gene for mobilization shows sequence similarities to Mob/Pre proteins found in Gram-positive bacterial plasmids. The rolling circle mechanism commonly used by small Gram-positive plasmids is not utilized; Rep is the gene involved in replication.

REPLICATES IN:
Acetobacter xylinum, Alcaligenes eutrophus, Bartonella bacilliformis, Bordetella spp, Brucella spp, Caulobacter crescentus, Escherichia coli, Paracoccus denitrificans, Pseudomonas fluorescens, Pseudomonas putida, Rhizobium meliloti, Rhizobium leguminosarum,Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae, Xanthomonas campestris.

Cat. No.	Description	Size/Vol.
5300-300	pBBR122 vector DNA	10 µg, lyophilized
Shipped at RT; store at 4°C