BIO 101:Products:Functional Analysis:Multiple Cloning Site Vector pMCS 5

Multiple Cloning Site Vector pMCS 5M
The Ultimate Multiple Cloning Site: Contains 59 unique restriction sites within the MCS

APPLICATIONS

standard cloning into a site up-stream from a T7 RNA Polymerase promoter
end-cloning
labeling
linearization of recombinant clones
blue/white selection
generation of single-strand DNA (using the f1 origin)

The new cloning vector pMCS5 contains the ultimate multiple cloning site (MCS) with 59 unique restriction sites. Included are the recognition sites for all commonly used restriction enzymes, providing a suitable cloning site for almost any application: 46 unique hexamer sites, 2 heptamer sites, and sites for all 10 known octanucleotide-specific endonucleases. In addition, the well-defined 18-mer sequence of the extremely rarely cutting enzyme, I-Sce I, is located at one terminus of the MCS, enabling the linearization of recombinant clones. I-Sce I does not recognize a palindrome and can thus be used for end labeling and cloning. Further, it can be utilized for strand protection in unidirectional deletion experiments. Also included in the MCS are restriction sites which occur very infrequently in human DNA, such as, Mlu I, Nru I and Spl I.

Recognition Sequences	Enzymes	Properties
Heptamer sequences	Rsr II, Bae I
Octamer sequences	Asc I, Fse I, Not I, Srf I, Sfi I Pac I, Swa I Pme I, SgrA I, Sse8387 I	only GC pairs only AT pairs
Hexamer sequences	Mlu I, Nru I, Spl I Cla I, Acc I, Acc65 I, Age I, Apa I, Avr II, Bbe I, BamH I, Bgl II, Bsp120 I, BspD I, BspM I, BssH II, Ecl136 I, EcoR I, EcoR V, Ehe I, Hind III , Hpa I, Kas I, Kpn I, Mun I, Nar I, Nco I, Nde I, Nhe I,Nsi I, Nsp7524 V, Pml I, Ppu10 I, Pst I, Sac I, Sac II, Sal I, Sma I, SnaB I, Spe I, Sph I, Xba I, Xho I, Xma I, Xma III	very infrequent in human DNA
18-mer sequence	I-Sce I (see ref 2)	highly unlikely in any DNA

List of Sites: Rsr II, Bae I Asc I, Fse I, Not I, Srf I, Sfi I, Pac I, Swa I, Pme I, SgrA I,
Sse83871, Mlu I, Nru I, Spl I, I-Sce I, Cla I, Acc I, Acc65 I, Age I, Apa I,
AvrI I, Bbe I, BamH I, Bgl II, Bsp120 I, BspD I, BspM I, BssH II, Ec1136 I,
EcoR I, EcoR V, Ehe I, Hind III, Hpa I, Kas I, Kpn I, Mun I, Nar I, Nco I,
Nde I, Nhe I, Nsi I, Nsp7524 V, Pml I, Ppu10 I, Pst I, Sac I, Sac II,
Sal I, Sma I, SnaB I, Spe I, Sph I, Xba I, Xho I, Xma I, Xma III

ORDER INFORMATION:
Cat. No.	Description	Amount
5301-110	Vector pMCS 5	5 痢
Shipped lyophilized at RT; store at 4°C

Restriction Enzymes: (Shipped on dry ice)		Recog. site
5301-111	I-Sce-I	18 mer
5301-112	Not I	8 mer
5301-113	Srf I	8 mer
5301-114	Rsr II	7 mer
5301-115	Bae I	7 mer

References:

Hoheisel, J.(1994) BioTechniques 17(3) 456-459.
Thierry et al.(1991) Nucl. Acid Res. 19, 189-190.

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