Lambda PS Eukaryotic Libraries
Easy-to-Handle Eukaryotic Lambda-PS Libraries
Eight frequently used genomic libraries with automatic subcloning capability.

APPLICATIONS

• screening
• easy characterization of clones
• homology studies
• gene targeting (mouse library: strain 129, congenic with popular ES cell lines)
• developmental, evolutionary, and genetic studies on vertebrate and non-vertebrate organisms

In comparison with other library systems, genomic libraries created in the well characterized vector lPS significantly increases the ease of screening and characterization of clones . The lPS system combines the advantages of large insert genomic libraries with the convenience of working with high copy plasmids. Since the l replacement vector can accommodate up to 20 kb DNA inserts, complex genomes are completely represented in a reasonable number of recombinants (approximately 5-fold coverage). For characterization of selected clones, the system provides for automatic plasmid subcloning of insert fragments: linear lPS phage contain two loxP sites in direct orientation flanking a high copy plasmid backbone and the insert. Recombination between these two sites is mediated by Cre recombinase (in the Cre-expressing E. coli strain BNN132). This leads to excision of the multi-copy plasmid from the phage genome obviating the need for subcloning the insert in another system. A restriction map is easily compiled using the excised plasmid, and the Bluescript backbone facilitates further manipulation of the plasmid DNA. Five vertebrate and three non-vertebrate libraries are offered at a very competitive price. One area of special is the library from the pufferfish genome (Fugu rubripes), which is 7.5 times smaller than the human genome. Because of its similar gene repertoire it is an excellent model for the discovery of human genes. The l-PS phage vector for the construction of custom libraries is also available for the experienced researcher.

Automatic subcloning: Site-specific recombination between loxP sites catalysed by Cre recombinase from E.coli BNN132.

ORDER INFORMATION
Cat. No.	Library	Size/Vol.
5300-910	1) human	1 ml
5300-911	2) mouse	1 ml
5300-912	3) rat	1 ml
5300-913	4) zebrafish	1 ml
5300-914	5) pufferfish	1 ml
5300-915	6) C. elegans	1 ml
5300-916	7) D. melanogaster	1 ml
5300-917	8) S. cerevisiae	1 ml
5300-918	vertebrate kit (1-5)	1 ml each
5300-919	non-vertebrate kit (6-8)	1 ml each
5300-920	complete kit (1-8)	1 ml each
5300-921	lambda-PS phage	1 ml
The lPS vector and libraries are shipped frozen (on "blue ice") as phage stock in 7% DMSO. Store at -70 or -20 C for long term. The host strains C600 and BNN132 and a detailed handbook are supplied with the libraries. The titers of the libraries are sufficiently high to perform >100 screens. Re-amplification is not advisable.