cycle SEQ Kits
Manual and Automated, Cycle Sequencing Kits from Display Systems Biotech using the Unique cycleSEQ Polymerase.

Whether you sequence DNA by manual or automated methods, cycle-sequencing technology offers advantages over non-cycle methods, not the least of which is the need for significantly less template DNA. End-labeled primer methods demonstrate decided advantages over other methods: Reactions primed by contaminating sequences will not be detected, which is very useful when working with templates that are difficult to obtain in pure enough form for use with other sequencing methods. Radiolysis of endlabeled primers produces unlabeled fragments that do not affect results, whereas radiolysis of internal labeling of reaction products can result in smeared bands and uninterpretable results.

Two cycle-sequencing Kits are offered from Display Systems Biotech:

1. The Manual cycleSEQ Kit:
   
   • This kit utilizes radiolabeled primers
   • Uses a new cycleSEQ enzyme for even band intensity
   • Effective sequencing of GC-Rich organisms or regions of high GC content.
   • Reagents are premixed into A, C, G and T reactions.
   • Easy, two step protocol:
     
     1. End-label primer.
     2. Aliquot each reaction and cycle 30 times.
2. The Automated cycleSEQ Kit.
   
   • Long-Read, High Fidelity DNA Sequencing (600-700 bases)
   • Uniform band intensity for easy detection of mixed sequences.
   • Uses a new cycleSEQ enzyme for even band intensity
   • Effective sequencing of GC-Rich organisms or regions of high GC content.
   • Reagents are premixed into A, C, G and T reactions.
   • Easy, one step protocol:
     
     1. Aliquot each reaction and cycle 30 times.