BIO 101:Products:Functional Analysis:Standard Cloning

Standard Cloning Vectors

Cloning Vectors
Cat. No.	Vector	Resistance	Ori	Speciality	Quantity
5300-300	pBBR122	C,K		see pg.128	10 痢
5301-110	pMCS 5	A	Col E1	see pg. 144	5 痢
5301-210	pBR322	T,A	Col E1	reading frame shift gives AmpR, TcS transformants	25 痢
5301-211					100 痢
5301-220	pBR325	T,A,C	Col E1	Cm resistant pBR322	25 痢
5301-221					100 痢
5301-230	pBR328	T,A,C	Col E1	non-mobilizable pBR325	25 痢
5301-231					100 痢
5301-240	pACYC184	T,C	p15A1	double-transformants with Col E1 vectors	25 痢
5301-241					100 痢
5301-250	pAT153	T,A	Col E1	non-mobilizable; higher copy than pBR322	25 痢
5301-251					100 痢
5301-260	pUC18	A	Col E1	MCS within lacZ: blue/white selection	25 痢
5301-261					100 痢
5301-270	pUC118	A	Col E1	M13 origin for ssDNA production	25 痢
5301-271					100 痢
Shipped lyophilized at RT; store at 4°C. A - ampicillin C - chloramphenicol K - kanamycin T - tetracycline

Bacillus megaterium
Highly Efficient Expression System

High yielding stable protein expression; suitable for large scale protein production.
E. coli/B megaterium shuttle vector pWH1520 contains MCS downstream of promotor for versitile cloning.
Expression tightly regulated by xylose operon; xylose inducible up to 350 fold.
No endotoxins.

References:

Rygus T. and Hillen, W. (1991) Appl. Microbiol. Biotechnol.35, 594.
Vary, P. (1994) Microbiology 140,1001.
Hueck, C.P. et al.(1995) Molecular Microbiology 16,855.

Bacillys megaterium Kit	5301-700
Kit contents: 5痢 pWH1520 (E. coli/B megaterium shuttle vector), lyophilized DNA.5 x 550 痞 B. megaterium (strain DSM319) protoplasts ready for 5 transformations; protocol included.
Separate Components: pWH1520 Vector DNA, 5痢 lyophilized DNA.	5301-701
DSM319 protoplasts, 5 x 550 痞	5301-702

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