Whole Cell Yeast PCR Kit

The WHOLE CELL YEAST PCR KIT is designed to release DNA (plasmids, YACs, and genomic DNA) from whole cells (colonies or liquid cultures) for PCR analysis. Using a proprietary enzyme mixx, the tough yeast cell wall is destroyed in a single short incubation and the resulting cell suspension is used directly in the PCR reaction. Such a treatment is essential to give reproducible PCR results without purifying the DNA first.

Optimal performance is obtained if Molecular Biology Grade yeast media from BIO 101 is used. This is particularly important if yeast liquid cultures are directly used without pelleting first.

Release plasmids, YACs and genomic DNA from yeast colonies for PCR analysis.

The protocol, in brief:

1. Add 10 ul WHOLE CELL LYSIS solution to yeast cells (2 µl overnight culture or a fraction of a colony)
2. Incubate at 37°C for 15 min
3. Use 2-5 ul for a 100 ul PCR reaction