ssPHAGE SPIN Kits

These kits contain all the necessary reagents for isolation of highly purified ssDNA from phage in one hour. The process is especially appropriate for rapid preparation of "long read" sequencing templates from ssDNA phage vectors. No enzymatic reactions or organic extractions are required in this protocol.

The kits contain all the necessary reagents and materials for 60, 120, and 600 minipreps. The miniprep protocol is summarized as follows:

1. Stab phage plaque and inoculate 1.5 ml media in a small culture tube. Grow phage for 8-16 hours.
2. Spin down 1.5 ml of the bacterial culture and remove 1 ml of supernatant.
3. Precipitate and pellet phage with ssPhage Drop Buffer.
4. By resuspending the phage pellet in ssPhage

ssDNA isolated by the ssPhage DNA SPIN Kit: Different sized inserts were cloned in an M13 vector and transformed into host cells. Plaques were picked at random and grown in culture overnight. One ml of each of the culture supernatants was treated according to the ssPhage DNA SPIN protocol and an aliquot of the eluted ssDNA was electrophoresed on a 1% agarose gel. The bands indicate that several different sizes of inserts were cloned.

ssPHAGE SPIN Kits
Cat. No.	Description	Size/Vol.
2065-200	ssPhage DNA Spin Kit	60 Preps
Kit Contents
2065-201	ssPhage Drop Buffer	26 ml
2065-202	ssPhage GLASSMILK SpinBuffer	26 ml
2065-203	ssPhage Wash Solution	12 ml
2065-204	Spin Filter with Tube	60
2065-205	Catch Tube	60
2065-400	ssPhage DNA Spin Kit	120 Preps
Kit Contents
2065-401	ssPhage Drop Buffer	50 ml
2065-402	ssPhage GLASSMILK SpinBuffer	31 ml
2065-403	ssPhage Wash Solution	24 ml
2065-404	Spin Filter with Tube	120
2065-405	Catch Tube	120
2065-600	ssPhage DNA Spin Kit	600 Preps
Kit Contents
2065-601	ssPhage Drop Buffer	251 ml
2065-602	ssPhage GLASSMILK SpinBuffer	155 ml
2065-603	ssPhage Wash Solution	120 ml
2065-604	Spin Filter with Tube	600
2065-605	Catch Tube	600