RPM® Yeast Plasmid Isolation Kit
Plasmid Isolation from Yeast
The RPM® Yeast Plasmid Isolation Kit is designed for the isolation of plasmid DNA from yeast cells. It uses GLASSMILK® purification in conjunction with Spin Filter technology to increase throughput and achieve optimal DNA quality. The isolated DNA can be used successfully in PCR screens and bacterial transformations. The same kit can be used to isolate plasmid DNA from recombinant bacteria for use in any downstream application (PCR, sequencing, restriction enzyme analysis, labeling, etc.) This dual purpose kit is ideal for screening putative positive yeast transformants obtained from two-hybrid screens.
DNA is eluted in H2O and is ready for use
No phenol/chloroform extractions
No alcohol precipitation
A brief outline of the protocol:
| RPM® Yeast Plasmid Isolation Kit | ||
|---|---|---|
| Cat. No. | Description | Size/Vol. |
| 2069-000 | RPM Yeast Plasmid Isolation Kit | 12 Preps |
| Kit Components | ||
| 2069-001 | Yeast Lysis Matrix | 12 tubes |
| 2069-002 | Alkaline Lysis Solution | 4 ml |
| 2069-003 | Neutralizing Solution | 4 ml |
| 2069-004 | GLASSMILK® Spin Buffer | 5 ml |
| 2069-005 | Wash Solution Concentrate | 7 ml |
| 2069-006 | SPIN Filters | 12 |
| 2069-007 | Catch Tubes | 12 |
| 2069-200 | RPM® Yeast Plasmid Isolation Kit | 25 Preps |
| Kit Components | ||
| 2069-201 | Yeast Lysis Matrix | 25 tubes |
| 2069-202 | Alkaline Lysis Solution | 8 ml |
| 2069-203 | Neutralizing Solution | 8 ml |
| 2069-204 | GLASSMILK® Spin Buffer | 8 ml |
| 2069-205 | Wash Solution Concentrate | 14 ml |
| 2069-206 | SPIN Filters | 25 |
| 2069-207 | Catch Tubes | 25 |
| 2069-400 | RPM®Yeast Plasmid Isolation Kit | 100 Preps |
| Kit Components | ||
| 2069-401 | Yeast Lysis Matrix | 100 tubes |
| 2069-402 | Alkaline Lysis Solution | 32 ml |
| 2069-403 | Neutralizing Solution | 32 ml |
| 2069-404 | GLASSMILK® Spin Buffer | 32 ml |
| 2069-405 | Wash Solution Concentrate | 55 ml |
| 2069-406 | SPIN Filters | 100 |
| 2069-407 | Catch Tubes | 100 |
