RPM® AFS
For Long-Read Templates
A Rapid Pure Midiprep Kit designed to produce template DNA for Automated Fluorescent Sequencing.
Yield up to 50 µg plasmid DNA per prep of high copy plasmid, and up to 15 µg plasmid from low copy host/plasmid systems designed to maximize insert stability.
As many investigators have determined, excellent long-read sequencing results can only be obtained consistently from medium scale template prep methods developed by BIO 101 and utilized in the RPM® AFS Kit.
The procedure, as detailed in the protocol section, uses proprietary wash steps to ensure isolation of long-read template DNA but is otherwise similar to that of the RPM SPIN Midi Kit described on the previous page.
BIND - WASH - ELUTE
All steps are done in a microcentrifuge using specially designed Spin Filters. The resulting DNA is eluted in a small volume of water and is ready for use.
| RPM® AFS Template preparation: Uncut plasmid template, lane 1; template DNA cut with Hind III, lane 2; template cut with Pst I, lane 3. Results of sequencing uncut template in lane 1 is shown in the lower figure on the facing page. |  |
| RPM® AFS KIT | ||
|---|---|---|
| Cat. No. | Description | Size/Vol. |
| 2072-200 | RPM® AFS Kit | 50 Preps |
| Kit Contents | ||
| 2072-201 | Pre-lysis Buffer | 12 ml |
| 2072-202 | Alkaline Lysis Solution | 24 ml |
| 2072-203 | Neutralizing Solution | 18 ml |
| 2072-204 | AFS GLASSMILK® | 30 ml |
| 2072-205 | Wash Solution Concentrate | 30 ml |
| 2072-206 | Spin Filter with Tube | 50 |
| 2072-207 | Catch Tube | 50 |
| 2072-208 | Elution Solution | 15 ml |
| 2072-400 | RPM® AFS Kit | 150 Preps |
| Kit Contents | ||
| 2072-401 | Pre-lysis Buffer | 36 ml |
| 2072-402 | Alkaline Lysis Solution | 72 ml |
| 2072-403 | Neutralizing Solution | 54 ml |
| 2072-404 | AFS GLASSMILK® | 90 ml |
| 2072-405 | Wash Solution Concentrate | 90 ml |
| 2072-406 | Spin Filter with Tube | 150 |
| 2072-407 | Catch Tube | 150 |
| 2072-408 | Elution Solution | 40 ml |
