RPM^® MonsterPrep Kit

The RPM^® 12G Kit makes use of the same methodology and protocol as the RPM^® 4G, but on a still larger scale. Use this kit for preparation of 10-15 mg of high copy plasmid DNA in two and a half hours from 12 g of cells from 1 to 3 liters of culture, depending upon media and growth conditions.

Plasmid DNA isolated with the RPM 12G Kit. The plasmid prep was dissolved in 1 ml of water and the concentration, as measured by UV, was 13 mg. The DNA was diluted 10 fold and 1, 2, 4, and 10 µl were run on the gel as shown above.	Plasmid DNA uncut and cut. 1 µl of plasmid DNA isolated with the RPM® 12G Kit, as shown in the left photograph, was cut with Eco RI, Hind III, and Pst I for 1 hour and separated on a gel, as shown above. Each enzyme has a single site in the plasmid and the illustration shows that the plasmid was linearized in each case.

Cat. No.	Description	Size/Vol.
2079-200	RPM® 12G Kit	4 Preps
Kit Contents
2079-201	Cell Wash Concentrate	17 ml
2079-202	Alkaline Lysis Concentrate I	34 ml
2079-203	Alkaline Lysis Concentrate II	27 ml
2079-204	Neutralizing Solution	260 ml
2079-205	GLASSMILK® 12G	4 x 40 ml
2079-206	Wash Solution Concentrate	90 ml
2079-207	RNase Mixx	2.2 ml
2079-208	5M NaCl	7.5 ml
2079-209	Binding Salt	4 x 53 g
2079-210	Bottle A	1
2079-211	Bottle B	1
2079-212	Filter Unit 12G	4
2079-213	DNA Trap Tube	4
2079-215	Pre-Lysis Concentrate	27 ml