MERmaid® Kit
For Rapid Isolation of OligoMERs from Gels and Solution


MERmaid employs a technology based on GENECLEAN®, but uses a different silica matrix known as GLASSFOG®  which binds smaller DNA fragments that are in the 10-200 bp range. This property makes the MERmaid® Kit the method of choice for purifying all types of double- and single-stranded DNA oligomers from solution or gels, e.g., small PCR products, primers, linkers, and other oligonucleotides.

The protocol is similar to that of GENECLEAN®:

  1. Add High Salt Binding Solution and GLASSFOG® to the solution or to the excised gel band and allow DNA to bind (5-10 minutes).
  2. Wash away non-bound reagents (5 minutes)
  3. Elute DNA from GLASSFOG® with water or TE (5 minutes)



Removal of Oligomers from Agrose

Purification of 12 and 14 base oligomers from 4% BioGel: Lanes 1 and 2 show 12 and 14 base oligomers before removal from the gel. Lanes 3 and 4 show the efficiency of recovery of 12 and 14 base oligomers from lanes 1 and 2. Estimated recovery is >75%.



Cat. No.DescriptionSize/Vol.
1005-200MERmaid® Kit200 Preps
Kit Contents
1005-201High Salt Binding Solution60 ml
1005-202Ethanol Wash Concentrate15 ml
1005-203BioGel (Sample)5 g
1101-206GLASSFOG®1.5 ml

Recovery of a 10 mer from solution and 3% BioGel agarose using the MERmaid® Kit: Lane 1 is a control containing the starting amount of oligomer. Lanes 2 and 3 are duplicate samples recovered from solution. Lanes 4 and 5 are duplicate samples removed from 3% BioGel agarose. 1
2
3
4
5



Protocols




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