G-NOME^® Mini MonsterPrep Kit

The G-NOME^® kit was developed by BIO 101 as a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, yeast, and animal cells and tissues of all types. No organic extractions are required, and up to 100 µg of DNA can be isolated per prep.

The protocol, briefly, is as follows:

1. Suspension of tissue or cells (homogenized if necessary).
2. Lysis of cells in the presence of RNase.
3. Incubation with protease.
4. Precipitation of digested proteins and other debris by proprietary "salting out" procedure, followed by centrifugation.
5. Addition of ethanol to supernatant, followed by isolation of precipitated DNA by spooling or centrifugation.

The kits come in three sizes, suitable for 10, 25, or 100 preparations. Each preparation starts with approximately 100 mg of cells or tissue and yields up to 100 µg of high MW genomic DNA.

Genomic DNA isolated from mouse, yeast, alfalfa, and bacteria using the G-Nome® DNA Isolation Kit. From the left lane of each DNA type is uncut, Hind III, and Mbo I cut. JM101 is: uncut, and cut with isoschizimers Mbo I, Dpn I and Sau3A, left to right. Mbo I does not cut DAM methylated JM101. DNAs were cut to completion for 40 minutes at 37°C and separated on an 0.7% Agarose gel.

G-NOME® DNA Kit
Cat. No.	Description	Size/Vol.
2010-200	G-NOME® DNA KITS	10 Preps
Kit Contents
2010-201	Cell Lysis/Denaturing Solution	1 ml
2010-202	RNase Mixx	0.6 ml
2010-203	Protease Mixx	0.3 ml
2010-204	Salt-Out Mixture	6 ml
2010-205	Cell Suspension Solution	20 ml
2010-400	G-NOME® DNA KITS	25 Preps
Kit Contents
2010-401	Cell Lysis/Denaturing Solution	2.5 ml
2010-402	RNase Mixx	1.4 ml
2010-403	Protease Mixx	0.8 ml
2010-404	Salt-Out Mixture	15 ml
2010-405	Cell Suspension Solution	50 ml
2010-600	G-NOME® DNA KITS	100 Preps
Kit Contents
2010-601	Cell Lysis/Denaturing Solution	10 ml
2010-602	RNase Mixx	5.5 ml
2010-603	Protease Mixx	3 ml
2010-604	Salt-Out Mixture	60 ml
2010-605	Cell Suspension Solution	200 ml
Shipping & Storage: Ambient/Except RNase Mixx,Protease Mixx: 4°C