FLORACLEAN^TM Kit
Quick and Easy Plant Chromosomal DNA Isolation

Because of the wide variety of plant tissue types and cytoplasmic non-DNA components, the most consistent results are obtained by first isolating nuclei and discarding non-nuclear fractions. Purified nuclei are lysed to obtain DNA free of phenolics, polysaccharides, and other contaminants that are often difficult to eliminate from DNA preps of plant cells and can inhibit subsequent enzymatic manipulation of the DNA. FLORACLEAN is a modification of the popular G-Nome DNA Isolation Kit that has been designed specifically for isolation of nuclear chromosomal DNA from plant tissues. No organic extractions are required, and up to 400 µg of DNA can be isolated from 1.5 g of tissue in 4-6 hours (more than half of which is incubation time). The isolated DNA is suitable for PCR or enzymatic manipulations.

The protocol in brief:

1. Homogenize tissue in a nuclear buffer.
2. Pellet and wash nuclei.
3. Lyse nuclei with detergent.
4. Incubate with protease.
5. "Salt-Out" digested proteins and other debris.
6. Spool or pellet precipitated DNA after addition of ethanol.

Nuclear DNA isolated from plant tissues
Genomic DNA from cornsilk and iceplant leaf. RAPD analysis of DNA isolated by FloraClean	Genomic DNA from cornsilk and iceplant leaf.

FLORACLEAN Kit
Cat. No.	Description	Size/Vol.
2012-200	FLORACLEAN Kit	10 Preps
Kit Contents
2012-201	Nuclear Buffer Concentrate	50 ml
2012-202	Nuclear Suspension Solution	25 ml
2012-203	Nuclear Lysis Solution	2 ml
2012-204	Protease Mixx	300 µl
2012-205	Salt-Out Mixture	6 ml
2012-211	CHO	43 g
2012-212	Nuclear Buffer Chaotrope	1.25 ml
5040-201	Sieve Material	1 sheet
2012-400	FLORACLEAN Kit	25 Preps
Kit Contents
2012-401	Nuclear Buffer Concentrate	125 ml
2012-402	Nuclear Suspension Solution	45 ml
2012-403	Nuclear Lysis Solution	5 ml
2012-404	Protease Mixx	700 µl
2012-405	Salt-Out Mixture	15 ml
2012-411	CHO	107 g
2012-412	Nuclear Buffer Chaotrope	3.12 ml
5040-201	Sieve Material	1 sheet
2012-600	FLORACLEAN Kit	100 Preps
Kit Contents
2012-601	Nuclear Buffer Concentrate	500 ml
2012-602	Nuclear Suspension Solution	200 ml
2012-603	Nuclear Lysis Solution	15 ml
2012-604	Protease Mixx	3 ml
2012-605	Salt-Out Mixture	55 ml
2012-611	CHO	428 g
2012-612	Nuclear Buffer Chaotrope	12.5 ml
5040-201	Sieve Material	1 sheet
Shipping & Storage: Ambient/Except RNase Mixx,Protease Mixx: 4°C