The FastPrep® System
The FastPrep® System: FP120 + FastDNA®/FastRNA® Kits



RNA Isolation:

A revolutionary method for isolation of undegraded RNA from virtually any source. The FastPrep® System employs a rapid vertical angular motion and combination of proprietary matrices and chaotropic reagents to simultaneously homogenize tissues, lyse cells, and stabilize RNA in seconds. This unique system was used to isolate high quality RNA from M. tuberculosis, S. pyogenes and S. aureus, three bacteria where isolation of RNA is very difficult by conventional methods. The FastPrep® System has been used to isolate RNA from more than 200 diverse sources including: bacteria, yeast, algae, botanical samples, insects, intact mammalian tissues, and cultured cells. In all cases, crude stable total RNA was obtained within 2 minutes and pure RNA was isolated in less than 30 minutes demonstrating wide application for this system.

DNA Isolation:

The use of the FastPrep® System has provided a means of DNA isolation from sources that are difficult or virtually impossible without the use of the rapid reciprocating motion of the FP120 Instrument. For example, PCRable DNA has been isolated from soil organisms, bone, hard or dried animal and plant tissue samples. The procedure is convenient and takes less than one hour to complete.

References:

  1. Cheung, A. L., Eberhardt, K.J. and Fischetti, V. A., Analytical Biochemistry, 222, 511-514, 1994.
  2. Dana, R. C., The Genetic Revolution, San Diego Conference, AACC, November, 1994.
  3. Tantod, B., Barnes, L., Dana, R.C., ASCB Meeting, San Francisco, December, 1994.
  4. Dana, R. C., Saghbini, M., Lippman, D., and Gautsch, J., Plant GNome III, San Diego, January, 1995.
  5. Dana, R. C., Saghbini, M., Lippman, D., and Cheung, A. L., J. NIH Research, 7:61, 1995.
  6. Chen, W., Gray, E. and Gray, R., Molecular Plant Pathology, Vol. 86, No. 10, 1996, P 1140-148. 7. Borneman, J., Skroch, P. W., O'Sullivan, K. M., Palus, J. A., Rumjanek, N. G., Jansen, J. L., Nienhuis, J. and Triplett, E. W., Applied and Environmental Microbiology, June 1996, p. 1935-1943
  7. Salas, S. D., Bennett, J. E., Kwon-Chung, K. J., Perfect, J. R., and Williamson, P .R.,The Journal of Experimental Medicine, Vol. 184, August 1996, p. 377-386.
  8. Cheung, A. L., Wolz, C., Yeaman, M. R., and Bayer, A. S., Journal of Bacteriology, June 1995, p. 3220-3226.
  9. Bej, A. K., N.G., W., Morgan, S., Jones, D. D., and Mahubani, M. H., Molecular Biotechnology, Vol. 5, 1996.
  10. Bej, A. K., Smith III, A. L., Vickery, M. C. L., Jones, D. D. and Mahubani, M. H., Food Testing and Analysis, December, 1996, January, 1997, p. 16-21.
  11. Eggermont, K., Goderis, I. J. and Broekaert, W. F., Plant Molecular Biology Reporter, 14 (3) 1996, p. 273-279.


The FastPrep® System: A benchtop instrument capable of efficient disruption of tissues and cellular material for a wide variety of applications. The unit is used with FastRNA® extraction kits and FastDNA® extraction kits.








Cat. No.DescriptionList
6000-121FastPrep® SYSTEM
FP120 Instrument, Including Optimization Kit plus choice of any FastRNA® or FastDNA® Kit.
6000-120FastPrep® INSTRUMENT
FP120 Instrument




Home ¦ Products ¦ Custom Services ¦ Protocols ¦ Catalog Request ¦ Map ¦ About BIO 101