FastDNA^® Kit
Isolation of genomic DNA from plant and animal tissues and cells.

The FastDNA^® Kit has been condensed to one inclusive kit. Researchers can now choose the lysing matrix to use for their individual applications. FastDNA^® tubes are preloaded with matrix and users can select between 4 different matrix combinations to efficiently homogenize their samples. A convenient table in the protocol is provided that shows 5 different samples and the yield, OD260/280, DNA size, and gel photo obtained using each of the 4 different matrix combinations. The table and gel photos are used as a guide to help you to decide which matrix combination to use for your specific application.

The kit includes 3 different chaotropic buffers for the homogenization of specific samples: animal tissues and bacteria, plants, and yeast and fungi. After sample homogenization, DNA is purified with a GENECLEAN^® procedure in 30 minutes. Resulting DNA is up to 25 kb in length and PCR ready.

The protocol in brief:

1. Choose desired matrix combination; add sample and Cell Lysis (CLS) Solution, and process in the FastPrep^® Instrument
2. Centrifuge sample and transfer supernatant to a new tube.
3. Add Binding Matrix and centrifuge. Wash Matrix with Salt/Ethanol Solution.
4. Elute DNA in ultrapure water.
5. DNA is ready for use.

Fast DNA® Kit
Cat. No.	Description	Size/Vol.
6540-400	FastDNA Kit®	100 Preps
Kit Contents
6540-401	Lysing Matrix	100 x 2 ml
6540-404	1/4" Cylindrical Beads	100 beads
6540-408	Binding Matrix*	66 ml
6540-405	SEWS-M**	12 ml
6540-406	DES	20 ml
6540-407	BBS	200 µl
Specific Applications:
Plants:
6540-402	CLS-VF	90 ml
6540-403	PPS	25 ml
Animal Tissue
6540-409	CLS-TC***	110 ml
Yeast, Algae, and Fungi
6540-411	CLS-Y	110 ml
Optional
2080-800	SPIN Modules	100
2080-600	SPIN Modules	60
2080-400	SPIN Modules	40
*Contains Guanidine Thiocyanate, Use with proper precaution. **Protocol for preparation of SEWS-M (salt/ethanol wash solution, DNase-free): Add 100 ml ethanol to the bottle labeled SEWS-M, which already contains 12 ml of DNase-free salt solution, to make a total of 112 ml. Shake and store tightly capped at room temperature. *** In case a precipitate is seen, heat bottle in 45-55°C water bath and allow to cool to room temperature.