FastDNA^® SPIN Kit for Soil
Isolation of DNA from soil organisms to assess microbial diversity and soil health

BIO 101 introduces a NEW kit for the study of microbial diversity of agricultural soils. The FastDNA^® SPIN Kit for Soil is used in conjunction with the FastPrep^® instrument to rapidly isolate and purify genomic DNA from soil organisms in only 30 minutes. Up to 12 samples can be processed in the FastPrep^® instrument simultaneously for convenient screening of the genetic diversity in test samples. The kit is characterized by its simplicity in efficiency in extracting DNA up to 25 kb in length from all bacteria, fungi, plants, and animals in a soil community. Resulting DNA is PCR amplification ready.

A brief outline of the protocol:

1. Add up to 500 mg of soil to the homogenization tube and add lysing reagents.
2. Process in the FastPrep^® Instrument and centrifuge tubes to pellet cellular debris and proteins.
3. To supernatant, add DNA Binding Matrix and transfer entire solution to a SPIN Filter.
4. Centrifuge SPIN Filter; wash DNA bound GLASSMILK^® with wash solution; and elute the DNA in water.
5. DNA is PCR ready.

FastDNA® SPINKit for Soil
Cat. No.	Description	Size/Vol.
6560-200	FastDNA® Kit for Soil	50 Preps
Kit Contents
6560-215	MULTIMIX 2 Matrix Tubes	50 tubes
6540-408	Binding Matrix	66 ml
6540-405	SEWS-M	12 ml
6540-406	DES	20 ml
6560-205	Sodium Phosphate Buffer	60 ml
6540-407	BBS	200 µl
6511-202	MT Buffer	8 ml
6560-210	SPIN Filters	50
6560-211	Catch Tubes	50