CsCl CIRCLEPREP^®
Step Gradient Kit

For preparation of plasmid DNA of the highest purity for research uses, banding in a cesium chloride gradient has long been considered the method of choice. Conventional CsCl-based preps, however, require several days. BIO 101's CsCl Circleprep^® Step Gradient Kit allows the entire procedure to be completed in one day.

After a one hour alkaline lysis procedure, the cleared lysate is put in a CsCl step gradient. Following an overnight centrifugation at 45,000 rpm, the purified band of plasmid DNA is removed and purified through a syringe cartridge purification system (see next product page, EZ Band Cleanup System) to remove CsCl and ethidium bromide. After elution from the cartridge, the purified DNA is precipitated and dissolved at the desired concentration for immediate use.

The main elements of the protocol are as follows:

1. Alkaline lysis, 1 hour.
2. Prepare step gradient, 30 minutes.
3. Overnight centrifugation.
4. EZ Band Cleanup, 30 minutes.

CsCl CIRCLEPREP® Step Gradient Kit
Cat. No.	Description	Size/Vol.
2009-200	CsCl Circleprep Kit (includes 1 x 2008-200)	12 Preps
Kit Contents
2009-201	Alkaline Lysis Solution I	55 ml
2009-202	Alkaline Lysis Solution II	40 ml
2009-203	Neutralizing Solution	400 ml
2009-204	CsCl DNA Solution	165 ml
2009-205	CsCl RNase MIXX	550 µl
2009-206	Crystalline DNase-free CsCl	55 g
2009-208	CsCl Liquid Solution	100 ml
2009-208	Ethidium Bromide Solution	5.5 ml
5040-202	Sieve Material	2 Sheets
2009-400	CsCl Circleprep® Kit (includes 1 x 2008-400)	24 Preps
Kit Contents
2009-401	Alkaline Lysis Solution I	110 ml
2009-402	Alkaline Lysis Solution II	80 ml
2009-403	Neutralizing Solution	2 x 400 ml
2009-404	CsCl DNA Solution	330 ml
2009-405	CsCl RNase MIXX	1.1 ml
2009-406	Crystalline DNase-free CsCl	110 g
2009-407	CsCl Liquid Solution	200 ml
2009-408	Ethidium Bromide Solution	11 ml
5040-204	Sieve Material	4 Sheets