BioPulverizer System
Homogenize Tissues and Cells in a Closed System

The BioPulverizer System consists of 2 ml screw-cap tubes that contain different sizes and shapes of specially prepared ceramic media for homogenizing cells and tissues. Samples are placed in the tube with appropriate reagents and the tube is shaken by hand or mechanical device to homogenize the sample and lyse cells. The tubes are ideally suited for cell disruption/homogenization where a closed system is necessary or advantageous because prior grinding or blending is not required. Depending on the makeup of the sample, homogenization in the BioPulverizer tubes can be done by hand or on a vortex mixer. Samples more difficult to lyse or homogenize can be shaken in a cell disruption device such as a Beadbeater or FastPrep^® Instrument.

General method for use of BioPulverizer tubes:

1. Add 10 to 100 mg of sample and appropriate reagents to tube and seal with screw cap.
2. Shake tube by hand or in disruption device until desired homogenization/lysis is achieved.
3. Centrifuge tube in a microcentrifuge to pellet debris. Remove supernatant and continue with appropriate protocol to process for isolation of desired component.

BioPulverizer System
Cat. No.	Description	Size/Vol.
6570-101	BioPulverizer Sampler (5 tubes each of 6570-201 to 6570-601)	25 Tubes
6570-201	BioPulverizer H Tubes contain: 6 mm ceramic sphere, 6mm ceramic rod, garnet matrix	50 Tubes
6570-301	BioPulverizer Blue Tubes contain: 0.1 mm silica spheres	50 Tubes
6570-401	BioPulverizer Red Tubes contain: 1.0 mm silica spheres	50 Tubes
6570-501	BioPulverizer Green Tubes contain: 1.4 mm ceramic spheres	50 Tubes
6570-601	MultiMix Tubes contain: Green and Blue Matrix with an additional 4 mm silica sphere	50 Tubes

BioPulverizer Tubes and matrix are RNase/DNase-free. Matrices are washed with hot water and detergent (and some types with acid), rinsed thoroughly, and baked in a 400°C oven for 24 hours.