Media Additives and Ingredients
| Sterile 80% Glycerol | ||
|---|---|---|
| Bacteria and yeast cells can be stored for months to years in a liquid or solid form at -10°C to -15°C, or frozen at -20°C to -80°C in 20 - 50% glycerol. Glycerol is added to fresh cells from liquid or plate cultures and stored in the freezer. Fresh media cultures are inoculated by dipping into the frozen stock with a sterile loop or pipet. The sterile 80% glycerol solution offered below is less viscous and more convenient to pipet than 100% glycerol. | ||
| Solution | ||
| 50 ml | 3055-024 | |
| 100 ml | 3055-034 | |
| 500 ml | 3055-044 | |
| 4 x 500 ml | 3055-054 | |
| Sterile 20% Glycerol | ||
|---|---|---|
| Solution | ||
| 100 ml | 3055-534 | |
| 500 ml | 3055-544 | |
| 4 x 500 ml | 3055-554 | |
| Glucose | ||
|---|---|---|
| D(+)glucose, FW180.2, (dextrose; corn sugar) white powder, tested for optimum growth when included in bacteria, slime mold, and yeast media formulations. | ||
| Powder | ||
| 227g (0.5 lb) | 3055-012 | |
| 454g (1.0 lb) | 3055-022 | |
| 1 kg (2.2 lb) | 3055-032 | |
| 5 kg (11.0 lb) | 3055-042 | |
| Galactose (Glucose free) | ||
|---|---|---|
| D(+)galactose, FW180.2, (grape sugar) white powder, <0.1% glucose. For use in defined yeast media where a glucose-free galactose is required. | ||
| Powder | ||
| 227g (0.5 lb) | 4008-012 | |
| 454g (1.0 lb) | 4008-022 | |
| 1 kg (2.2 lb) | 4008-032 | |
| 5 kg (11.0 lb) | 4008-042 | |
| Raffinose | ||
|---|---|---|
| D(+)raffinose pentahydrate, 99%, FW594.5, white powder. For use in defined yeast media. | ||
| Powder | ||
| 227g (0.5 lb) | 4010-012 | |
| 454g (1.0 lb) | 4010-022 | |
| 1 kg (2.2 lb) | 4010-032 | |
| 5 kg (11.0 lb) | 4010-042 | |
| Sucrose | ||
|---|---|---|
| C12H22O11, FW342.3, 99% (cane or beet sugar, saccharose), white, small crystalline. For use as carbon source in certain yeast media formulations. | ||
| Powder | ||
| 227g (0.5 lb) | 4013-012 | |
| 454g (1.0 lb) | 4013-022 | |
| 1 kg (2.2 lb) | 4013-032 | |
| 5 kg (11.0 lb) | 4013-042 | |
| Casamino Acids | ||
|---|---|---|
| Acid hydrolyzed casein; rich in amino acids except tryptophane. Excellent as a supplement for rich media. Also see AHC Medium pg. 297. | ||
| Capsules | ||
| 227g (0.5 lb) | 3060-011 | |
| 454g (1.0 lb) | 3060-021 | |
| 1 kg (2.2 lb) | 3060-031 | |
| 5 kg (11.0 lb) | 3060-041 | |
| Powder | ||
| 227g (0.5 lb) | 3060-012 | |
| 454g (1.0 lb) | 3060-022 | |
| 1 kg (2.2 lb) | 3060-032 | |
| 5 kg (11.0 lb) | 3060-042 | |
| Yeast Extract-B | ||
|---|---|---|
| A dried and powdered, water-soluble extract of autolysed yeast; rich in B vitamins, amino acids and peptides. An autoclaved solution of Yeast Extract-B is crystal clear and provides optimum nutrients for bacterial growth; used in most BIO 101 media formulations. | ||
| Capsules | ||
| 227g (0.5 lb) | 3065-511 | |
| 454g (1.0 lb) | 3065-521 | |
| 1 kg (2.2 lb) | 3065-531 | |
| 5 kg (11.0 lb) | 3065-541 | |
| 15 kg (33.0 lb) | 3065-551 | |
| Powder | ||
| 227g (0.5 lb) | 3065-512 | |
| 454g (1.0 lb) | 3065-522 | |
| 1 kg (2.2 lb) | 3065-532 | |
| 5 kg (11.0 lb) | 3065-542 | |
| 15 kg (33.0 lb) | 3065-552 | |
| Yeast Extract-B, Granulated | ||
|---|---|---|
| The same as the powdered form of Yeast Extract-B above, but formed into small granules which makes it easier to handle than the fine powder form which can cause irritation of the breathing passages if inhaled. | ||
| Granulated Powder | ||
| 227g (0.5 lb) | 4023-012 | |
| 454g (1.0 lb) | 4023-022 | |
| 1 kg (2.2 lb) | 4023-032 | |
| 5 kg (11.0 lb) | 4023-042 | |
| 15 kg (33.0 lb) | 4023-052 | |
| Tryptone-B | ||
|---|---|---|
| Tryptone-B is a dried and powdered pancreatic digest of casein. The bacteriological grade has been chosen for optimum growth of bacteria and is used in BIO 101's bacterial media formulations. | ||
| Capsules | ||
| 227g (0.5 lb) | 3066-011 | |
| 454g (1.0 lb) | 3066-021 | |
| 1 kg (2.2 lb) | 3066-031 | |
| 5 kg (11.0 lb) | 3066-041 | |
| 15 kg (33.0 lb) | 3066-051 | |
| Powder | ||
| 227g (0.5 lb) | 3066-012 | |
| 454g (1.0 lb) | 3066-022 | |
| 1 kg (2.2 lb) | 3066-032 | |
| 5 kg (11.0 lb) | 3066-042 | |
| 15 kg (33.0 lb) | 3066-052 | |
| Agar-B (Molecular Biology Grade for Bacteria) | ||
|---|---|---|
| Agar-B is a bacteriological grade agar of the highest quality. After autoclaving, a solution of 1.5% Agar-B is a light amber color completely free of insoluble particulates. Medium agar plates poured with 1.5% Agar-B solidify into a haze-free, firm-surface gel perfect for inoculating, streaking, or replica plating cells in the manner commonly practiced by the molecular biologist. Agar-B was chosen as demonstrating superior qualities from among a large number of agar samples from different sources after testing for the ability to promote bacterial colony growth (colony diameter after 24 and 48 hours) and phage growth (plaque diameter and morphology of both M13 and Lambda bacteriophages). Agar-B is supplied in a light tan-colored, small granular form and is also packaged in capsules and pouches to fit the particular needs of the researcher. | ||
| Capsules | ||
| 227 g (0.5 lb) | 3072-011 | |
| 454 g (1.0 lb) | 3072-021 | |
| 1 kg (2.2 lb) | 3072-031 | |
| Powder/Small Granular | ||
| 227 g (0.5 lb) | 3072-012 | |
| 454 g (1.0 lb) | 3072-022 | |
| 1 kg (2.2 lb) | 3072-032 | |
| 5 kg (11.0 lb) | 3072-042 | |
| 15 kg (33.0 lb) | 3072-052 | |
| Pouch, 0.5 liter (7.5 g): | ||
| 1 ea | 3072-015 | |
| pack (10 x 1 ea) | 3072-065 | |
| case (10 x packs) | 3072-085 | |
| Pouch, 1 liter (15 g): | ||
| 1 ea | 3072-025 | |
| pack (10 x 1 ea) | 3072-075 | |
| case (10 x packs) | 3072-095 | |
| Marine Peptones | ||
|---|---|---|
| Peptones derived from fish; liquid form. No bovine or mammalian components. Excellent for formulating growth/fermentation media. Bulk quantities available, please inquire for details. | ||
| "Holy Mackerel 1" | ||
| 100 ml | 4017-014 | |
| 1 liter | 4017-024 | |
| "Holy Mackerel 2" | ||
| 100 ml | 4017-114 | |
| 1 liter | 4017-124 | |
| "Holy Mackerel 3" | ||
| 100 ml | 4017-214 | |
| 1 liter | 4017-224 | |
| "Holy Mackerel 4" | ||
| 100 ml | 4017-314 | |
| 1 liter | 4017-324 | |
| Noble Agar | ||
|---|---|---|
| A purified agar that is completely nutrient-free that is most often used in defined media formulations where traces of nutrients cannot be tolerated. | ||
| Powder | ||
| 227 g (0.5 lb) | 3073-012 | |
| 454 g (1.0 lb) | 3073-022 | |
| 1 kg (2.2 lb) | 3073-032 | |
| 5 kg (11.0 lb) | 3073-042 | |
| 15 kg (33.0 lb | 3073-052 | |
| X-GAL | ||
|---|---|---|
| 5-bromo-4-chloro-3-indolyl-b-D-galactoside Ultra Pure, Molecular Biology Grade; substrate for ß-galactosidase; forms intense blue color. | ||
| 1 g | 4063-102 | |
| 5 g | 4063-112 | |
| IPTG | ||
|---|---|---|
| Isopropylthio-ß-galactoside: induces ß-galactosidase activity in bacteria. Use with X-GAL. Ultra Pure, Molecular Biology Grade. | ||
| 1 g | 4064-102 | |
| 5 g | 4064-112 | |
| 5-FOA | ||
|---|---|---|
| 5-Fluoroorotic Acid Added to the media when working with yeast to select for Ura-; if itŐs Ura+ it will die in the presence of 1 mg/ml 5-FOA. [Boeke, J.D., et al. (1987)Meth. Enzymol.154,164]. Slightly soluble in water. Sold as a powder or sterile 25x solution. | ||
| 1 g | 4066-102 | |
| 10 ml | 4066-104 | |
| 100 ml | 4066-114 | |
| 3-AT | ||
|---|---|---|
| 3-amino-1,2,4-triazole is a competitive inhibitor of the HIS3 gene product in yeast. When 3-AT is added to yeast media (1-10mM) it will limit histidine biosynthesis and growth; used in some two-hybrid screens to "fine tune" leaky expression of the HIS3 reporter gene. | ||
| 50 g | 4061-722 | |
| 500 g | 4061-742 | |